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Hands in the Soil

METABARCODING

Multi-Species Identification

the large-scale taxonomic identification of complex (environmental) samples.

Metabarcoding

Metabarcoding is a technique of species identification using DNA/RNA from several different organisms can be extracted from either bulk samples or environmental samples. DNA collected from environmental samples is used for environmental DNA analysis. eDNA includes DNA analysis using metabarcoding methods to assess the biodiversity of environmental samples.

Barcoding and Metabarcoding

The Difference of

The main difference between barcoding and metabarcoding is that metabarcoding does not focus on one specific organism, but instead aims to determine species composition within a sample. Differences in the standard methods for DNA barcoding and metabarcoding. While DNA barcoding focuses on a specific species, metabarcoding examines whole communities.

Application of Metabarcoding

Metabarcoding can be used to reconstruct ancient ecosystems from DNA found throughout the world, to explore the interconnectness between plants and pollinators, to assess diet without need for feeding observation or stomach flushing, to detect invasive species before they could possibly be detected via traditional means, to determine community responses to pollution, and even to assess air quality and its implications for human health.

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Metabarcoding Workflow

DNA
Extraction

Collection Samples

DNA
Amplification

NGS
Sequencing

Bioinformatics
Analysis

Taxonomic
Assignment

Sample
Collection

Customer needs to send a bulk samples or environmental samples. The quality of DNA in the sample influences the results of our analysis, for this reason all samples should be stored frozen or dry (especially water samples is using a membrane filter should be stored in an appropriate medium).

 

We suggest you to get in touch with us to receive support in planning your sampling, fixing and storing your samples.

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